Repopulation of Decellularized Human Adipose Extracellular Matrices with host adipose cells
Fabiana Zanata, MD, PhD1, Caasy Thomas-Porch, PhD2, Jie Li, PhD3, Elizabeth C. Martin, PhD4, Nicholas Pashos, PhD2, Kaylynn Genemaras, Master student2, Nick Poche, PhD4, Nick Totaro, PhD4, Melyssa R. Bratton, Master student5, Dina Gaupp, Histology Core2, Trivia Frazier, PhD2, Xiying Wu, PhD6, Wei-dong Tian, PhD3, Guangdi Wang, PhD3, Bruce Bunnell, PhD2, Lauren Flynn, PhD7, Daniel Hayes, PhD4, Jeffrey M. Gimble, MD, PhD2, Lydia M. Ferreira, MD, PhD, Full Professor1.
1Universidade Federal de Sao Paulo, Sao Paulo, Brazil, 2Tulane University, New Orleans, LA, USA, 3West China School of Stomatology, Sichuan, China, 4Louisiana State University, Baton Rouge, LA, USA, 5Xavier University of Louisiana, New Orleans, LA, USA, 6LACell LLC, New Orleans, LA, USA, 7Western University, London, United Kingdom.
Purpose: Since surgeons use human adipose tissue for tissue grafting to improve volume and contour, the promise of a processed biological scaffold could bring a new approach with a product commercially available for immediate use. Adipose tissue decellularization has been accomplished using enzymatic and/or detergent methods. Methods: To examine the hypothesis that decellularization processes may yield adipose scaffolds, the current study employed in vivo implantation into GFP+ transgenic mice of enzymatic and detergent based decellularized scaffolds. Samples of each group of enzymatic and detergent decellularized scaffolds were implanted in the subcutaneous area in the back of nine animals in triplicates using silk scaffold as a control. The scaffolds were removed after three, six or nine weeks post-transplantation for histologic (Hematoxylin and Eosin and Masonís Trichrome staining) and immunohistochemistry (GFP and Perilipnin) analysis. Results: The decellularized samples showed an adipose like aspect different from the silk controls that were fibrotic and less regular. Histologic examination confirmed the macroscopic findings. Functional adipose cells from the host were shown by GFP positive cells and Perilipin staining. The repopulation of the scaffolds with the host viable adipose cells increased with the time after transplantation. The findings supported the ingrowth of host-derived adipocytes and vasculature in a time dependent manner. Conclusion: These results determine that decellularization methods of adipose tissue-derived bioscaffolds maintain its ability to promote ASC adhesion, proliferation and differentiation into mature adipose tissue in an in vivo animal model.
Back to 2018 Posters