Analyzing Tropism Between Adipose Derived Stem Cells and Breast Cancer Cells of Different Malignancies
Matthias A. Sauter1, Elizabeth A. Brett, MSc1, Christina M. Müller, BSc2, Hans-Günther Machens, Professor1, Dominik Duscher, Doctor1.
1Technical University Munich, Munich, Germany, 2Ludwig Maximilian University of Munich, Munich, Germany.
PURPOSE:Many regenerative therapies rely on the infiltration of tissue/synthetic constructs with stem cells. The adipose derived stem cell has received appropriate scientific and clinical attention, given its multipotency and easy procurement. However, in the surgical world of breast cancer reconstruction and fat grafting, attraction of ASCs to the treatment zone is viewed as an oncogenic risk. The current scientific standard however does not yet fully illustrate the role of ASCs in breast cancer recurrence. This study aims to add new insights on the trophic effect of ASCs towards breast cancer cells.
METHODS:Silicon chambers were used to seed isolated populations of cells in the same well of a cell culture dish. Once cell populations had adhered, chambers were removed and cells were allowed to follow natural trophic cues. Multiple permutations of MDA-MB-231, MCF-7, HS-27, and ASCs were engineered. Cells were stained with MitoTracker for fluorescent visualization. Cellular tropism was qualitatively analyzed photographically, and quantitatively measured by pixel densiometry in Image J. Metabolic assays were performed to quantify activity of the cell populations while migrating in vitro.
RESULTS:The present series of novel co-culture systems reveal that ASCs do not migrate faster towards a benign cancer (MCF-7) when compared synchronously to fibroblasts. Conversely, in a model with aggressive breast cancer cells (MDA-MB-231), ASCs are seen to have a highly pronounced tropism to the malignant cancer population which remains static. Simultaneously, in the same model, cancer cells exhibit significant migration towards a static fibroblast population (p< 0.05). The attraction of ASCs to MDA-MB-231 cells is dose dependent, showing higher migration for higher breast cancer cell numbers.
CONCLUSION:Taken in totality, these data show for the first time the attraction of ASCs to malignant breast cancer cells, compared to benign; a phenomenon which many ASC studies infer. This study should extend research to questioning what exact role (inducer, catalyst, inhibitor etc.) ASCs play with different types of breast cancer.
Back to 2019 Abstracts