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Oxygen Sensing Liquid Bandage: A Novel Approach in Tissue Perfusion Assessment
Pieter G. L. Koolen, M.D.1, Zongxi Li, PhD2, Marek A. Paul, MD1, Thomas Huang, BA1, Ahmed Ibrahim, MD, PhD1, Conor L. Evans, PhD2, Samuel J. Lin, MD, MBA1.
1Beth Israel Deaconess Medical Center / Harvard Medical School, Boston, MA, USA, 2Wellman Center for Photomedicine / Harvard Medical School, Boston, MA, USA.

PURPOSE:
Oxygenation status and early detection of vascular compromise are fundamental in the prevention of postoperative flap failure after microsurgical breast reconstruction. Various non-invasive and invasive modalities have emerged as an adjunct to standard clinical monitoring, however, no current method is ideal. In this study we propose a novel, non-invasive approach which allows for assessment of tissue perfusion by using a newly developed oxygen sensing paint-on dye incorporated into commercially available liquid bandage matrix material.
METHODS:
A thin film of porphyrin based, oxygen sensing dye was applied to the skin surface of eight rats. The infrarenal aorta was dissected and clamped at one minute intervals for 20 minutes, and subsequently unclamped. Signal intensity was captured by a camera based imaging device in order to create a two-dimensional mapping of skin tissue oxygenation. Near infrared imaging and a Clark electrode were used as control methods.
RESULTS:
Clamping the aorta resulted in a rapid decrease in oxygenation (see Figures). More importantly, registered oxygenation demonstrated similar pattern among groups along with recovery.
CONCLUSION:
This proof-of-concept study demonstrates the capacity of oxygen sensing films to accurately detect underlying tissue oxygenation and provides a basis for further development of non-invasive tissue oxygenation assessment.


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