Non-Thermal Plasma: Safe, Versatile Technology for Device Sterilization and Wound Healing Applications
Kerry A. Morrison, BA1, Xue Dong, BA1, Ope A. Asanbe, MD1, Eleonora Kierkels, BA1, Omer Kaymakcalan, MD1, Yoshiko Toyoda, BA1, Ross Weinreb, BA1, Czeslaw Golkowski, PhD2, Jason A. Spector, MD, FACS1.
1Laboratory of Bioregenerative Medicine & Surgery, Weill Cornell Medical College, New York, NY, USA, 2SteriFreeMed, Inc., Cornell University, Ithaca, NY, USA.
SteriFreeMed Plasma Processing Technology (SFM) is a novel, non-thermal plasma (NTP)/free radical, portable device that delivers a highly active reactive oxygen and nitrogen species (RONS) mixture within a closed loop system, which rapidly and reliably sterilized cell phones after a single 10-minute NTP treatment, without residual effects on phones. Given the device’s remarkable disinfection efficacy, we sought to investigate the potential applicability to living tissue.
Two 8mm, full-thickness, splinted excisional wounds were created on C57bl/6 mice. Mice were treated once for 10 minutes, and sacrificed after 7 or 14 days. LIVE/DEAD assays were performed on human umbilical vein endothelial (HUVEC), human aortic smooth muscle (HASMC), human fibroblast foreskin (HFF1), and human placental pericyte (HPLP) cells in single and co-culture following 10-minute NTP treatments.
After NTP treatment, there was no gross or histological evidence of residue, aberrant dermal architecture, infection, or edema. By Day 7, treated wounds demonstrated statistically smaller wound widths than controls (4.8mm±0.3mm vs. 7.1mm±0.4mm, p<0.01). LIVE/DEAD assays demonstrated 100% cell viability after 10-minute NTP treatments for both single culture and co-cultured combinations of all cells.
These data indicate that non thermal plasma may not only be safely used on living cells and tissue, but that it may even augment the wound healing process, indicating the potential for therapeutic application in the biomedical field. SFM has the potential to revolutionize the current practice of device sterilization in the hospital setting as well as to shift the paradigm of wound healing.
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