Topical Minocycline Effectively Decontaminates and Reduces Inflammation in Porcine Wounds
Lauren T. Daly, MD, David M. Tsai, MD, Cameron CY Lee, BS, Raquel A. Minasian, BA, Elizabeth Kiwanuka, MD PhD, Florian Hackl, MD, Andrew B. Onderdonk, PhD, Johan PE Junker, PhD, Elof Eriksson, MD PhD, EJ Caterson, MD PhD.
Brigham & Women's Hospital, Boston, MA, USA.
Topical antibiotics have potential to treat wound infection and inflammation while minimizing adverse effects associated with systemic antibiotics.
Full-thickness wounds were created on four pigs, then randomized for Staphylococcus aureus infection. Using polyurethane wound enclosure devices, wounds were treated with 100 μg/mL minocycline, 1000 μg/mL minocycline or saline control. Bacteria were quantified in wound tissue and fluid obtained over 9 hours. Immunosorbent assays were used to analyze wound fluid concentrations of IL-1β, IL-6, TNF-α, matrix metalloproteinase-9 (MMP-9). Inflammatory cells were quantified in wound tissue using immunohistochemistry.
After 6 hours, 100 and 1000 μg/mL minocycline decreased bacteria in wound tissue to 3.5±0.87 and 2.9±2.3 log CFU/g respectively, compared to 8.3±0.9 log CFU/g in control wounds (p<0.001). After 2 hours, minocycline reduced inflammatory cytokines IL-1β, IL-6, and TNF-α concentrations (p<0.01). Wound tissue inflammatory cell counts decreased following 1 hour (p<0.05). In non-infected wounds, minocycline significantly reduced IL-1β, IL-6, and inflammatory cell counts after 4 hours (p<0.01). MMP-9 concentrations decreased after 1-hour treatment (p<0.05).
Topical minocycline rapidly decontaminates infected wounds while significantly reducing local inflammation. The ability of minocycline to reduce inflammation exists even independent of its antibacterial effect, suggesting it’s potential to improve wound healing and reduce scarring.
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