Plastic Surgery Research Council
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PSRC 60th Annual Meeting
Program and Abstracts

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Adipose-Derived Stem Cells Improve Engraftment of Full Thickness Skin Grafts by Increasing Angiogenesis
Michael Hu, MD, MPH, MS, Wan Xing Hong, MS, Ted Zhu, N/A, Zeshaan Maan, MD, Min Hu, MD, PhD, Kshemendra Senarath-Yapa, MD, Andrew Zimmermann, BS, Michael Chung, MD, Graham Walmsley, BS, Dominik Duscher, MD, Anna Luan, BS, Derrick Wan, MD, Peter Lorenz, MD, Michael Longaker, MD, MBA.
Stanford, Stanford, CA, USA.

PURPOSE:
Autologous full thickness skin grafts are one of the most common procedures used for wound closure. However, in diabetic patients skin graft rejection is common because of poor circulation around the graft. Herein, we examine the ability of adipose-derived stem cells (ASCs) to mediate acceptance of autologous skin grafts in both wild type and diabetic mice.
METHODS:
ASCs were harvested from inguinal fat pads of transgenic FVB-L2G mice. A 6 mm full thickness excisional wound was created on the dorsum of wild type and diabetic FVB mice. The right ear of each mouse was harvested and used as an autologous skin graft. Wounds were treated with or without ASCs (5 x 105) via injection under the wound bed. ASC survival was quantified in vivo through bioluminescent imaging (BLI). The effectiveness of the treatments was recorded over a period of two weeks.
RESULTS:
ASCs were found to promote acceptance of the autologous skin grafts in diabetic mice. All wild type mice demonstrated successful engraftment of skin grafts without ASC treatment. Diabetic mice with ASC treatment achieved 100% engraftment whereas only 20% of grafts were taken in control diabetic mice (*p<0.03). BLI revealed survival of ASCs when assessing for engraftment two weeks following wounding. Immunofluorescence showed an increase in VEGF and CD31 in diabetic FTSGs treated with ASCs versus no ASC treatment.
CONCLUSION:
We demonstrate improved engraftment of full thickness skin grafts in diabetic mice with therapeutic ASCs. This cell-based application may improve the efficacy of skin grafting in both diabetic and nondiabetic patients.


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