Plastic Surgery Research Council
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PSRC 60th Annual Meeting
Program and Abstracts

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Vascularized Bone Marrow Transplantation As A New Approach For Bone Marrow Transplantation
Yingjun Su, MD, PhD, Jianwu Chen, MD, Shuzhong Guo, MD, PhD.
Department of Plastic and Reconstructive Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, China.

PURPOSE:
A whole set of components of the bone marrow can be transferred to the recipients in the vascularized bone marrow transplantation (VBMT) such as in some vascularized composite allografting procedures. However, whether the VBMT is able to facilitate the long-term recovery of hemopoietic and lymphoid lineages is not clear, therefore, becomes the focus of this study.
METHODS:
5-Gy irradiated Lewis rats received either homologous bone marrow cells through intravenous infusion or the whole set of bone marrow components through the free femur graft transplantation. The transplanted femur grafts were placed in the recipients’ inguinal regions and their nutrient vessels were anastomosed with recipient vessels. The peripheral blood and bone marrow cellular profiles were characterized at different time intervals to assess the long-term recovery of hematopoietic and lymphoid lineages in the myeloablative recipients underwent VBMT or bone marrow cell infusion procedure.
RESULTS:
In this transplantation model, all femur grafts survived indefinitely and the bone marrow maintained normal morphologically at 100 days post-transplant. Compared with irradiated rats, both VBMT and bone marrow cell infusion reconstituted the recipients’ hemopoietic and lymphoid lineages. Furthermore, VBMT accelerated the recovery of complete hematopoietic population and maintained the normal bone marrow histology of the femur grafts. Similarly, the spleen index in VBMT group was much higher than that in control animals.
CONCLUSION:
Vascularized femur transplantation may provide an alternative method for bone marrow transplantation and can be used as a research tool to aid the study of roles of bone marrow in vascularized composite allografts.


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