Plastic Surgery Research Council
Members Only  |  Contact  |  PSRC on Facebook
PSRC 60th Annual Meeting
Program and Abstracts

Back to 2015 Annual Meeting Program


9-Cis-Retinoic Acid Ameliorates Surgically Induced Lymphedema In The Mouse Hindlimb Model
Athanasios Bramos, MD, Young-Kwon Hong, Ph.D., Alex K. Wong, M.D..
Keck School of Medicine of USC, Los Angeles, CA, USA.

PURPOSE:
Lymphedema is an incurable condition characterized by tissue swelling after lymphatic damage. Recently, we have shown that retinoic acids (RA) induce lymphangiogenesis independently from angiogenesis in vitro and in vivo and propose that RA therapy may minimize lymphedema development after surgical injury. In the present study we sought to investigate the effect of 9-cis- retinoic acid in a combined injury model of mouse hindlimb lymphdedema.
METHODS: Eight to ten week old Balb/c mice were radiated with 20 Gy in the right hindlimb and nine days later a circumferential skin incision was made in the right groin in order to excise the popliteal lymph node and associated lymphatic vessels. Animals were randomized to 0.8mg/kg/day of 9-cis-RA or vehicle control after surgery. Change in paw thickness and hindlimb volume relative to the un-operated left hindlimb was measured every 5 days for 45 days.
RESULTS:All mice developed extremity swelling and increased paw thickness in the operated hindlimb by post-operative day (POD) 5. Sustained lymphedema was observed in control treated animals for 45 days. At all time points, 9-cis-RA treated animals had decreased paw thickness relative to controls with statistically significant differences of >12% noted at POD 40 and 45 (p<0.05).
CONCLUSION: This study shows for the first time that administration of systemic 9-cis-RA has a protective role in a combined radiation and surgery induced lymphedema mouse model. Since 9-cis-RA is FDA-already approved for other clinical conditions, this data may facilitate rapid translation to human protocols.


Back to 2015 Annual Meeting Program