Plastic Surgery Research Council
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PSRC 60th Annual Meeting

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The Effect of Fat Harvest Technique on Adipocyte Viability and Adipose-Derived Stem Cell Count
Ava Hosseini, MD1, Brian Mailey, MD1, Jennifer Baker, MD1, Zeni Alfonso, PhD2, Paula Strasser, BS2, Kevin Hicok, PhD2, Steven Cohen, MD1, Amanda Gosman, MD1, Marek Dobke, MD, PhD1, Anna Wallace, MD1.
1University of California, San Diego, San Diego, CA, USA, 2Cytori Therapeutics, San Diego, CA, USA.

PURPOSE: Retrieval of viable adipose-derived stem cells (ADSC) in lipoaspirate may be an important determinant in positive long-term outcomes after autologous fat transfer; however, it is unknown if harvest technique alters lipoaspirate properties. We sought to evaluate the impact of 3 harvesting techniques on adipocyte viability and cell characterization of the isolated stromal vascular fraction (SVF).
METHODS: We compared patients who underwent autologous fat transfer to the breast from a prospectively accrued database using three different techniques. A sample of the lipoaspirate from each patient was collected and analyzed for cell yield and viability utilizing a NucleoCounter. Cell surface marker expression of SVF was characterized via flow cytometry and resolved into four different subpopulations. Outcomes were compared by harvest technique.
RESULTS: Twenty-one patients were accrued to the study. Of those, 7 underwent harvest utilizing hand-operated syringe-aspiration, 7 via water-assisted liposuction technique, and 7 via conventional machine liposuction. There was no difference in cell viability, number of colony forming units (CFU), or cells per gram of lipoaspirate between harvest techniques. The frequency of cell subpopulations identified by cytofluorometric characterization was also similar between groups. Importantly, there was no difference in the proportion of the CD34+/CD31-/CD45- population in SVF (27.3% vs 27.5% vs 27.5%; p=0.99) in the hand-syringe, water-assisted, and conventional machine liposuction respectively.
CONCLUSION: The ADRC cell count, cell viability, CFU, or proportions of SVF subpopulations are not affected by conventional fat harvest technique. Future work can determine if washing and purifying lipoaspirate may affect laboratory and clinical outcomes.


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