Plastic Surgery Research Council
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PSRC 60th Annual Meeting

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Novel Antimicrobial Coating Of Non-crosslinked Acellular Porcine Dermal Matrix Provides Protection From Microbial Colonization By Common Pathogenic Microorganisms In A Rabbit Model
Thomas A. Imahiyerobo, Jr., M.D.1, Jeffrey Scott, Ph.D.2, Jason A. Spector, M.D., F.A.C.S.1.
1Weill Cornell Medical College, New York, NY, USA, 2Brown University, Providence, RI, USA.

Novel antimicrobial coating of non-crosslinked acellular porcine dermal matrix provides protection from microbial colonization by common pathogenic microorganisms in a rabbit model
Authors: Thomas A. Imahiyerobo, M.D., Jeffrey Scott, Ph.D., Jason A. Spector M.D., F.A.C.S.
Abstract
Purpose: The objective of this study was to evaluate the antimicrobial efficacy of a novel antimicrobial (rifampin/minocyline)-coated non-crosslinked acellular porcine dermal matrix device (AM-coated ADM), as compared to Strattice™ Reconstructive Tissue Matrix (Strattice™) following inoculation with Methicillin-resistant Staphyloccus aureus (MRSA) or Escherichia coli (E.Coli) in a dorsal rabbit model.
Methods: 20 male New Zealand White Rabbits were bilaterally implanted with AM-coated ADM (n=10 rabbits) and Strattice™ (n=10 rabbits) respectively. Each device location was then inoculated with clinically-isolated MRSA (5x107 CFU/ml) (n=5 devices/group) or E.Coli (1x107 CFU/ml) (n=5 devices/group) using remotely tunneled polyethylene catheters. At 14 days post-implantation, each implant site was analyzed for abscess formation, and viable MRSA/E.Coli colony forming units (CFU) on and surrounding each device.
Results: Gross necropsy demonstrated significantly higher abscess scores for Strattice™ (MRSA: 3/3 score; E.Coli: 1.8/3 score), as compared to the AM-coated ADM (MRSA: 0/3 score; E.Coli: 0/3 score). Whereas Strattice™ demonstrated significant microbial colonization of the device (MRSA: 1.79 x 107 ± 4.88 x 106 CFU; E.Coli: 1.47 x 105 ± 7.70 x 104 CFU) and a greater percentage of positive pocket swabs in the medial and lateral surrounding tissues (MRSA: 10/10=100%; E.Coli: 7/10 = 70%), the AM-coated ADM and the surrounding tissues were devoid of bacterial colonization (device: MRSA: 0 ± 0 CFU; E.Coli: 0 ± 0 CFU; medial and lateral surrounding tissues: MRSA 0/10=0%; E.Coli: 0/10 = 0%).
Conclusions: AM-coated ADM completely inhibited device/surrounding tissue abscess formation and microbial colonization following inoculation with clinically-isolated MRSA and E.Coli in a dorsal rabbit
model. In contrast, Strattice™ demonstrated significantly greater MRSA and E.Coli microbial colonization, and marked to moderate abscess formation respectively. These data suggest that the AM-coated ADM device, unlike Strattice™, was able to protect itself and the surrounding tissues from MRSA and E.Coli microbial colonization, which may be advantageous in the setting of complex soft tissue reconstruction of the abdominal wall.


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