Plastic Surgery Research Council
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THE EFFECT OF ADIPOSE-DERIVED STEM CELLS AND PLATELET-RICH PLASMA FOR THE REPAIR OF THE CALVARIAL BONE DEFECT IN A MURINE MODEL
Presenter: Hiroshi Mizuno, MD
Co-Authors: Tajima S; Tobita M; Orbay H; Hyakusoku H
Juntendo University

Introduction: Previous studies have indicated that adipose-derives stem cells (ASCs) with growth factors and scaffolds may be promising for bone regeneration. Platelet-rich plasma (PRP) therapy represents an interesting biological technique to provide tissue repair by inducing chemotactic, proliferative and anabolic cellular responses. We have previously demonstrated that ASCs with PRP could generate both periodontal tissue and alveolar bone regeneration (Tobita et al Tissue Eng). The purpose of this study was to evaluate the ability of calvarial bone regeneration with ASCs and PRP.

Materials and Methods: The cultured ASCs isolated from inguinal fat pads of the Fischer rat were mixed with PRP obtained from inbred rats before implantation. Bone defect (?5mm) was made at the left calvarial bone with a dental round bar. The animals were randomly assigned to 4 groups (n=8 each) as following; Group 1: ASCs with PRP implantation group, Group 2: ASCs with Type I collagen gel implantation group, Group 3: PRP implantation group, Group 4: Type I collagen gel implantation group. After 4 and 8 weeks, micro-CT images were taken to assess the newly formed bone in the defect. Then the specimens were harvested and stained with hematoxylin and eosin (HE) and Alcian Blue.

Results: Micro-CT analysis showed that the surface area of newly formed tissue at 4 and 8 weeks were 95% and 95% in Group 1, 75% and 65% in Group 2, 50% and 55% in Group 3 and 44% and 55% in Group 4, respectively. Moreover, three-dimensional volume of the generated tissue at 4 and 8 weeks were 49% and 65% in Group 1, 39% and 33% in Group 2, 23% and 28% in Group 3 and 15% and 24% in Group 4, respectively. Trabecular bone-like structures was histologically evident particularly in Group 1. Alcian Blue staining was positive in Group 1 at both 4 and 8 weeks.

Conclusion: These findings suggest that ASCs and PRP could accelerate calvarial bone regeneration in a murine model. Since both ASCs and PRP can be of autologous and no biomaterials were used as scaffolds, such strategy may be relatively effective with safe for future clinical application.


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