Plastic Surgery Research Council
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DIFFERENTIAL INFLAMMATORY SIGNALING AND SYNTHESIS OF COLLAGEN AND FIBRONECTIN BY ADIPOSE STEM CELLS AND FIBROBLASTS OF DIFFERENT PHENOTYPES RELEVANT TO OUTCOME OF CELL-BASED WOUND THERAPIES
Presenter: Tianbing Yang, PhD
Co-Authors: Cetin-Ferra S; Tano Z; Barsic M; Dohar JE; Hebda PA
University of Pittsburgh

Based on previous studies in our rat skin wound healing model that adipose stem cells (ASCs) and fetal skin fibroblasts of embryonic day 15 (E15, scarless phenotype) significantly increased the regain of tensile strength (p<0.001, p=0.007, respectively), while fetal skin fibroblasts of embryonic day 18 (E18, scarring phenotype) and adult skin fibroblasts show minimal benefit, phenotypic and functional features of the four cell types are under investigation to explore the cellular and molecular mechanisms contributing to the quality of healing/scarring. Cell surface vimentin was detected as 6% positive for adult fibroblasts, 24% for E18 fibroblasts, 32% for E15 fibroblasts, and 31% for ASCs by flow cytometry, indicating significantly lower levels in adult fibroblasts by comparison with the other cell types. However, intracellular vimentin staining showed no differences among the four cell types (range of 95-99% positive). Intracellular staining also revealed no difference in collagen type I, but collagen type III and fibronectin shared a similar pattern with higher intracellular expression in ASCs and E15 fibroblasts compared to adult fibroblasts. The collagen secretory ability of the four cell types was evaluated by Western Blot and showed that both collagens type I and type III were only detectable in conditioned low serum medium of E15 and E18 fibroblasts, but not in ASCs or adult skin fibroblasts. Western blots also demonstrated a lower NFkBp65 expression in response to IL-1? only in E 18 fibroblasts, indicating a transition in inflammatory signaling at the wound healing developmental switch point (from regenerative to reparative) of E18. IkBa, an important NFkB inhibitor, increased in E18 fibroblasts, corresponding to the decreased level of NFkBp65. These results may help elucidate differences in cell-based wound therapies with implications for regenerative healing with reduced scar formation.

ACKNOWLEDGEMENTS
This work was supported in by the Armed Forces Institute for Regenerative Medicine (AFIRM), Wake Forest University of Pittsburgh consortium.


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